More Information

I'm investigating haploid fertility through the following approaches:

a) Establishing the interactome of AtPS1 using TurboID, a promiscuous biotin ligase. AtPS1 is a protein involved in spindle fiber orientation during meiosis, and its knockout results in parallel spindle formation, leading to the production of unreduced gametes and the restoration of haploid fertility.

b) Developing a computer vision model to detect Zea mays stomata in light-microscopy images and predict cell ploidy. This enables the analysis of cell patterning in haploids undergoing spontaneous genome doubling.

c) Screening candidate genes for haploid female fertility in Zea mays. A GWAS panel identified candidates, and haploids are being induced for fertility screening using T-DNA insertional mutants for each gene.